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Part:BBa_K2150019

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7+pTet+RBS+tetX-GFP(fusion protein)+DT

Two promoters are used, a constitutive promoter pTet and a T7RNAP specific promoter pT7. The fusion protein is tetX and GFP with a 3*GGGGS linker so that the expression level of tetX can be accurately reported by the fluorescence intensity of GFP. By testing the fluorescence intensity, we found that in the absence of T7 promoter, these two promoters exhibit higher efficiency than single pTet; the fluorescence intensity is lower that BBa_K2150018, in which the fusion protein is substituted by single tetX. With pT7, expression of GFP can be amplified while with pTet, expression of GFP can be repressed by tetR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1967


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